Discover how easy it is to grow mushrooms! Easy and quick to set up, liquid inoculation consists of depositing a liquid culture on a substrate. The mycelium will propagate during the incubation phase. It is then possible to move on to the fruiting phase to pick the mushrooms.
In this tutorial, we are going to learn how to use a mycelium syringe in liquid culture. One of the prerequisites is therefore to have a syringe of liquid culture of the mushroom variety of your choice. It is possible to provide it on the link following. Tutorial produced in partnership with the company Breizh Bell, a company in myciculture and low-tech production of organic mushrooms, in France.
Introduction to the mushroom cycle
What is commonly called "mushroom" is in fact only the temporary and visible "fruiting body": the sporophore (formerly called "carpophore").
It is the result of an organism with a more durable and more discreet character, formed of filaments generally invisible to the naked eye when they are isolated: the A9lium mycelium.
The sporophore is often in the form of a foot wearing a hat .
To cultivate a mushroom, we try to reproduce the natural external conditions. Mushroom cultivation is divided into three main stages.
In myciculture, inoculation by liquid culture consists of injecting mycelium contained in sterilized sugar water onto a substrate.
Why is it awesome?
There are several ways to start a mushroom crop. Liquid inoculation is one of the easiest because you just need to spray a substrate with a liquid culture. In addition, the risk of contamination is less than with other methods.
Receiving the liquid inoculation syringe
Upon receipt of the syringe containing the mycelium, place it immediately in the refrigerator, it must be used within two weeks!
You have two choices :
Mycelium: filamentous body of the fungus
Inoculation: injection of the mycelium into the substrate
Liquid culture: mixture of sterilized sugar water with myceliumSubstrate: Food of the fungus (grains, straw, wood...), the mycelium attaches to the substrate
(Liquid Mycelium syringe: Namekos, blue oyster mushroom, gray oyster mushroom, yellow oyster mushroom, pink oyster mushroom, shiitake mushrooms, red wine stropharia, etc.)
Equipment for liquid culture:
It is very important to have the most sterile conditions possible, throughout the manipulations. Indeed, there is a biological struggle between living species. If the environment is contaminated, the fungus will find itself in competition with other organisms, so it will be too fragile and will lose. We will seek to create ideal growing conditions to promote the fungus.
We advise you to place yourself in your kitchen, under your hood. It will prevent dust from settling on your crops.
We strongly advise you to handle your liquid mycelium out of sunlight, under a soft and weak light (night light for example).
In this step, we will make a jar for your liquid culture.
The lid of the jar will have two holes. They will be filled with two plugs.
The first stopper is self-closing and will allow the insertion of the syringe needle. The second is a filter cap for the respiration of the mycelium in the jar.
Your jar is ready for liquid inoculation!
- Follow step 1 of this tutorial.
The mycelium will spread.
Your culture is good when your jar is filled with a cloud of mycelium! The slightest green, blue or black stain in your jar is a sign of contamination and your culture will then be unusable.
When the mycelium has spread well, we invite you to carry out an inoculation on the grains. The steps are described below
If you want to inoculate grains, they will have to be cleaned on the first day.
- Boil water in your saucepan.
The liquid inoculation is carried out on the clean substrate. Either the substrate is in a jar (as in the pictures) or the substrate is contained in a grow bag. In both cases, the mycelium must be able to breathe.
If you choose a jar, pierce its lid and clean it with 70° alcohol. Then place a sterile compress in the hole.
(Be careful that the filter/compress does not touch alcohol)
(you will be able to see if your mycelium is colonizing your grains correctly after a few days, but be careful with the light!).
The mycelium will spread.
Your crop is ready when your beans turn completely white. The slightest green, blue or black stain in your jar is a sign of contamination and your culture will then be unusable.
As soon as your grains are colonized, you have the following two options:
Attention, the following steps are only valid for oyster mushrooms!
This step will allow the already stronger mycelium to settle on a harder substrate. Oyster mushrooms are fond of sawdust from hardwoods or straw. You can also combine the two.
Natural (non-odorized) rabbit litter is an ideal substrate and easy to find in stores!
In your kitchen, clean your worktop with alcohol.
If there is a bag, place it in a box isolated from light.