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Avant de faire vos achats, nous vous conseillons fortement de lire en entier le tutoriel. Nous vous encourageons à réaliser des achats groupés pour éviter le gaspillage et partager les éventuels frais de livraison.</translate> | Avant de faire vos achats, nous vous conseillons fortement de lire en entier le tutoriel. Nous vous encourageons à réaliser des achats groupés pour éviter le gaspillage et partager les éventuels frais de livraison.</translate> | ||
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{{Warning|La lame du scalpel ne doit pas toucher l’enveloppe externe du pleurote, celle-ci n’est pas stérile}} | {{Warning|La lame du scalpel ne doit pas toucher l’enveloppe externe du pleurote, celle-ci n’est pas stérile}} | ||
− | * Gardez le morceau de champignon sur le scalpel, ouvrez le couvercle d'une des boîtes de Pétri, et placez le morceau de champignon au centre de la gélose (voir photo 5) | + | *Gardez le morceau de champignon sur le scalpel, ouvrez le couvercle d'une des boîtes de Pétri, et placez le morceau de champignon au centre de la gélose (voir photo 5) |
− | * Refermez la boîte de Pétri et sceller avec le parafilm pour éviter les contaminations par l’air. Appliquez une extrémité du parafilm sur un bord de la boîte de Pétri puis tirez le parafilm pour le déposer sur toute la circonférence (voir photo 5). Il faut que le parafilm recouvre bien l'ouverture créée par le couvercle (voir photo 6) | + | *Refermez la boîte de Pétri et sceller avec le parafilm pour éviter les contaminations par l’air. Appliquez une extrémité du parafilm sur un bord de la boîte de Pétri puis tirez le parafilm pour le déposer sur toute la circonférence (voir photo 5). Il faut que le parafilm recouvre bien l'ouverture créée par le couvercle (voir photo 6) |
− | * Placez vos boîtes de Pétri dans un environnement sombre et ventilé comme un placard. Le développement du mycélium prendra quelques jours | + | *Placez vos boîtes de Pétri dans un environnement sombre et ventilé comme un placard. Le développement du mycélium prendra quelques jours |
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+ | }} | ||
+ | {{Tuto Step | ||
+ | |Step_Title=<translate>Préparation des grains</translate> | ||
+ | |Step_Content=<translate>Lorsque le mycélium s'est bien développé dans les boîtes de Pétri (voir photo 1), vous pouvez préparer les grains avant inoculation. Pour rappel, ce tutoriel est prévu pour réaliser 3 sacs de 1,7 kg de mycélium en grains chacun. Adaptez les indications selons le nombre de sac que vous souhaitez réaliser. | ||
+ | |||
+ | |||
+ | Pour chacun de sacs de culture : | ||
+ | |||
+ | *Versez 1,4 kg de grains de seigle | ||
+ | *Versez … g de chaux éteinte | ||
+ | *Versez 800 mL d'eau | ||
+ | *Mélangez le tout en seconouant le sac délicatement | ||
+ | *Fermer le sac avec une pince dédiée (voir photo 2) ou des pinces à ligne en bois | ||
+ | *Laissez reposer pendant 10 à 12h maximum</translate> | ||
+ | |Step_Picture_00=Biosph_re_Urbaine_-_Cl_nage_de_champignons_Capture_d_e_cran_2024-10-23_a_10.24.01.png | ||
+ | }} | ||
+ | {{Tuto Step | ||
+ | |Step_Title=<translate>Inoculation des grains de seigle</translate> | ||
+ | |Step_Content=<translate>* Nettoyez les surfaces de travail avec de l'alcool à 70°C, allumez la lampe à alcool et désinfectez le scalpel à l'alcool puis en passant sa lame dans la flamme afin de la stériliser | ||
+ | * Découpez la gélose en plusieurs morceaux puis versez le tout dans le sac de grains | ||
+ | * À travers le sac, décomposez la gélose et mélangez le tout pour la répartir humiformément dans les grains | ||
+ | *</translate> | ||
}} | }} | ||
{{Notes | {{Notes |
Tutorial de Biosphère Expérience | Catégories : Habitat, Alimentation
Ce tutoriel a été créé dans le cadre de l'expérience de mode de vie low-tech en ville menée par Biosphère Expérience à Boulogne Billancourt. Il présente les étapes de confection de mcyélium en grains par la filière dédiée à la culture de champignons.
Ce tutoriel a été créé dans le cadre de l'expérience de mode de vie low-tech en ville menée par Biosphère Expérience à Boulogne Billancourt. Il présente les étapes de confection de mcyélium en grains par la filière dédiée à la culture de champignons.
Le projet - Biosphère Urbaine
Pendant 4 mois, Corentin et Caroline ont expérimenté un mode de vie low-tech en ville en collaboration avec un réseau d'acteurs locaux. L'idée est de profiter de la forte densité de population pour répartir les connaissances, diviser le travail et mutualiser le matériel. Ainsi, Emma a constitué et coordonné des filières temporaires composées de structures locales mais aussi de citoyens. Au total, ce sont 14 personnes du territoire de Boulogne Billancourt qui ont participé à cette expérience !
Filière - culture de pleurotes
Les champignons sont délicieux et nutritifs, mais leur coût élevé sur le marché pose souvent un défi financier. Pour résoudre ce problème, Emma a créé une filière temporaire de culture de pleurotes qui assure tout le cycle de reproduction. À partir de pellets de paille et de mycélium en grains confectionnés au ChampiLab, Viriginie, Laurine, Corentin et Caroline ont reconstitué tout le cycle de culture du pleurote. L’idée est de mutualiser un espace pour créer des ateliers de confection des tours de culture mais aussi pour stocker le matériel et les tours pendant la phase d'incubation. Ce tutoriel vise à démocratiser la culture de pleurotes à l'échelle d'un territoire.
Déroulé de l'organisation durant l'expérience :
Ceci est une liste de matériaux exhaustive. Si vous avez déjà certains éléments, nous vous conseillons de favoriser la seconde main et d’adapter les dimensions et tailles tout au long du tuto.
Quantité pour réaliser 8 sacs de mycélium en grains ?
Ceci est une liste d'outils exhaustive à adapter en fonction de ce que vous possédez déjà.
Suivant le montage que vous choisissez et votre utilisation du réemploi, nous estimons entre 10 et 20 euros le coût pour réaliser un sac de mycélium en grains. Cela dépend de...
Avant de faire vos achats, nous vous conseillons fortement de lire en entier le tutoriel. Nous vous encourageons à réaliser des achats groupés pour éviter le gaspillage et partager les éventuels frais de livraison.
Stérilisation à la cocotte minute avec manomètre
Stérilisation à l'autoclave
Le clônage est une étape délicate car elle nécessite un espace propre et stérile pour éviter les contaminations. Dans le cadre du projet, nous avons monté un ChampiLab, un atelier partagé où l'on retrouve tout le matériel nécessaire pour réaliser du clônage. Notamment, nous avons fabriqué un flux laminaire avec l'entreprise Breizh Bell, spécialiste de la culture de champignons dans une démarche low-tech (voir tutoriel Biosphère Urbaine - ChampiLab). Le clônage peut se réaliser avec toute variété de champignons, idéalement pas trop vieux : un jeune champignon aura une croissance plus rapide. Dans le cadre du projet nous avons généralement clôner des pleurotes.
Question ? Comment on fait pour les autres boîtes de Pétri ? Il faut plsuieurs champignons ou pas ? Combien ?
Lorsque le mycélium s'est bien développé dans les boîtes de Pétri (voir photo 1), vous pouvez préparer les grains avant inoculation. Pour rappel, ce tutoriel est prévu pour réaliser 3 sacs de 1,7 kg de mycélium en grains chacun. Adaptez les indications selons le nombre de sac que vous souhaitez réaliser.
Pour chacun de sacs de culture :
Document rédigé par Emma Bousquet-Pasturel dans le cadre de programme de sciences participative de Biosphère Expérience.
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