Translations:Champignon Inoculation d'une culture liquide/64/en : Différence entre versions

(Page créée avec « - Follow step 1 of this tutorial. »)
 
Ligne 1 : Ligne 1 :
- Follow step 1 of this tutorial.
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* Follow step 1 of this tutorial.
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* Boil water in your pan.
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* Take your previously prepared jar (see step 2), fill it with boiled water and unrefined beet sugar (volume 4% of the volume of water). Close your jar, cover it with aluminum foil, emphasizing the opening area of ​​the lid.
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* Fill your pot with water again and boil it.
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* Place your wrapped jar in your saucepan and leave it covered for 2 hours (sterilization).
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* In your kitchen, turn on your hood and clean your worktop with alcohol.
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* Place tealights all around your work area.
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* Light the candles and take your syringe of liquid mycelium out of the refrigerator, clean (alcohol 70°) and place it in the center of the work area.
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* Take your syringe and needle out of their bag. Insert the needle.
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* Take your jar of liquid culture, clean the outside of this jar with 70° alcohol and place it in your work area.
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* Take two sterile compresses and soak them in alcohol.
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* Place the needle of your syringe on the flame of one of the candles and wait for the tip to turn red.
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* Then place it between the two compresses soaked in alcohol.
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* Completely empty the mycelium from your syringe into your jar.
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* Wrap your jar with aluminum foil, fabric or paper
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* Place it in an environment at 20°C and wait about two weeks. You can shake your jar so that your mycelium spreads even more and take the opportunity to see its evolution
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{{Warning|Watch out for the light!}}

Version du 29 avril 2022 à 08:58

  • Follow step 1 of this tutorial.
  • Boil water in your pan.
  • Take your previously prepared jar (see step 2), fill it with boiled water and unrefined beet sugar (volume 4% of the volume of water). Close your jar, cover it with aluminum foil, emphasizing the opening area of ​​the lid.
  • Fill your pot with water again and boil it.
  • Place your wrapped jar in your saucepan and leave it covered for 2 hours (sterilization).
  • In your kitchen, turn on your hood and clean your worktop with alcohol.
  • Place tealights all around your work area.
  • Light the candles and take your syringe of liquid mycelium out of the refrigerator, clean (alcohol 70°) and place it in the center of the work area.
  • Take your syringe and needle out of their bag. Insert the needle.
  • Take your jar of liquid culture, clean the outside of this jar with 70° alcohol and place it in your work area.
  • Take two sterile compresses and soak them in alcohol.
  • Place the needle of your syringe on the flame of one of the candles and wait for the tip to turn red.
  • Then place it between the two compresses soaked in alcohol.
  • Completely empty the mycelium from your syringe into your jar.
  • Wrap your jar with aluminum foil, fabric or paper
  • Place it in an environment at 20°C and wait about two weeks. You can shake your jar so that your mycelium spreads even more and take the opportunity to see its evolution
Watch out for the light!