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− | + | * Follow step 1 of this tutorial. | |
+ | * Boil water in your pan. | ||
+ | * Take your previously prepared jar (see step 2), fill it with boiled water and unrefined beet sugar (volume 4% of the volume of water). Close your jar, cover it with aluminum foil, emphasizing the opening area of the lid. | ||
+ | * Fill your pot with water again and boil it. | ||
+ | * Place your wrapped jar in your saucepan and leave it covered for 2 hours (sterilization). | ||
+ | * In your kitchen, turn on your hood and clean your worktop with alcohol. | ||
+ | * Place tealights all around your work area. | ||
+ | * Light the candles and take your syringe of liquid mycelium out of the refrigerator, clean (alcohol 70°) and place it in the center of the work area. | ||
+ | * Take your syringe and needle out of their bag. Insert the needle. | ||
+ | * Take your jar of liquid culture, clean the outside of this jar with 70° alcohol and place it in your work area. | ||
+ | * Take two sterile compresses and soak them in alcohol. | ||
+ | * Place the needle of your syringe on the flame of one of the candles and wait for the tip to turn red. | ||
+ | * Then place it between the two compresses soaked in alcohol. | ||
+ | * Completely empty the mycelium from your syringe into your jar. | ||
+ | * Wrap your jar with aluminum foil, fabric or paper | ||
+ | * Place it in an environment at 20°C and wait about two weeks. You can shake your jar so that your mycelium spreads even more and take the opportunity to see its evolution | ||
+ | {{Warning|Watch out for the light! Do not expose the mycelium to light during the incubation phase}} |
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