Elevage de Mouches Soldats Noires/en

Low-tech Lab

https://wiki.lowtechlab.org/wiki/Elevage_de_Mouches_Soldats_Noires/en

Dernière modification le 02/01/2020

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Description

This tutorial aims to facilitate the understanding of the life cycle of the black soldier fly and to learn how to grow it for domestic use. This technology was documented during the stopover of the Nomade des Mers expedition in Malaysia. The company EntoFood, which we visited, has been doing research on these flies for nearly 8 years and is preparing to launch its large-scale production unit. It will allow the treatment of 300 tonnes of organic waste per day!

Sommaire

Insectes, BSF, Compost, Déchets organiques, Protéines animales, Mouche soldat noir, Black soldier fly, NomadeDesMers en fr 1

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Introduction

Waste management, particularly in urban areas, is considered as one of the most important environmental issues for the coming years. The recycling of organic waste (bio-waste) is still quite limited although they represent more than a third of our garbage. Today, most of this organic waste, although recoverable, is buried or incinerated, bringing major environmental problems (pollution of the soil, air and groundwater, demand for increasingly large storage areas, etc.). The strong growth of urban populations makes it a major challenge for municipalities and more and more solutions are being tested.

An increasingly common solution is the conversion of organic waste by insects or larvae, including those of the black soldier fly (Black Soldier Fly, BSF): Hermetia illucens. This solution has attracted a lot of attention over the last decade for its speed of waste treatment as well as for the promising possibility of using harvested BSF larvae as a source of protein for animal feed, thus offering a valuable alternative to conventional feed (fishmeal in particular)

Whether on a medium or small scale, the breeding of black soldier fly larvae requires very few resources and makes it possible to effectively treat bio-waste by transforming it into a compostable and hyper-nutritive residue for the soil. In addition, larval recovery is possible to feed domestic animals (ducks, chicken, geese, fish...).

In summary, this are the advantages of growing BSF:

• The larvae are composed of ±40% protein and ±30% crude fat. This insect protein is of high nutritional quality and can be an interesting resource for animal feed (chickens, geese, ducks, fish...)
• Larvae have been shown to neutralize most disease-transmitting bacteria, such as Salmonella spp or E. coli, thus limiting the risk of disease transmission to animals and humans. [1]
• A reduction in the wet mass of organic waste of between 50 and 80%.
• The residue, a substance similar to compost, contains nutrients and organic matter that can be used directly on crops.
• Livestock farming is inexpensive and does not require sophisticated means of production. This makes it an accessible solution in all regions of the world.
• The black soldier fly (BSF) can be found in nature worldwide in tropical and subtropical regions between latitudes 40°S and 45°N

Youtube

Matériaux

The materials used will depend on the support used to build the living space of your larvae. This can be done in a wooden box, a drum cut in half, a large plastic box...

For the boat, we chose a wooden box because we had recovery boards

For an order of size, a box of ----- per ----- will allow to treat ---- kg of waste per week

For the box (120/65/30cm):

• 120/65cm x2 wood boards
• 120/30cm x2 wood boards
• 65/30cm x2 wood boards
• Iron angles
• Hinges
• Screw
• Paint and varnish
• Plastic tray of 100/60/15cm
• Plank and pieces of wood to make the ramp
• Inner tube
• Staples
• Plastic box to collect larvae

For the aviary (80/50/45cm):

• Wood bars of 80/5/2cm x4
• Wood bars of 41/5/2cm x2
• Wood bars of 40/5/2cm x2
• 50/45cm plywood board (for the bottom)
• Hinges
• Screw
• Angles
• Mosquito net
• 50/10cm board (for opening)

For pupae and egg laying:

• Cardboard or wooden plates separated by pins
• A box to put some waste
• A box to put the pupae
• Dry substrate (coconut fiber...)
• A box for the hydration of flies

Don't forget to protect the living space from the water. The eggs will burst with water!

Outils

The tools will also depend on the living space, for the construction of the box you will need:

• An electric or hand saw
• A hole saw
• Drill/screw or screwdriver
• Stapler
• Sander
• Brushes and rollers

Étape 1 - Construction of the breeding box

The breeding environment can be made with various materials. For the prototype of the boat for example, we built a wooden box in which we put a plastic bin containing the waste and larvae (See photo). For example, you can use a plastic drum cut on top, or a simple plastic box.

For the construction:

1. Preparation of the boards

• Using an electric or hand saw, cut the 6 boards to the right lengths
• Sand the boards
• Apply an undercoat on all sides of the boards and allow to dry
• Apply a coat of wood paint and let it dry
• Apply a coat of varnish (optional) and let it dry

2. Assembly of the box

• Using the angles and screws, assemble the boards on the bottom of the box
• Using angles and screws, connect the different sides of the box
• Cut out cleats to strengthen the inside of the box and attach the lid to it.
• Using screws, fix the cleats on the upper part of the walls

• Prepare the cover by installing hinges.
• Install the cover on the box and fix the hinges

3. Preparation of the waste box

If the sides of your waste bin are not very high, it may be useful to put an additional barrier against larvae escape

• Pass an old used inner tube all around your tray and leave a part coming out towards the inside
• Staple the inner tube to the tray
• Place the tray at the bottom of the box and stick it against one side

4. Preparation of the slope

• Cut the board in the shape of a trapezoid so that it forms a ramp when placed on the waste and joins the edge of the box at an angle of less than 45 degrees
• Cut cleats to fit the external shapes of the ramp
• Secure them with screws on the ramp

5. Installation of the ramp

To facilitate maintenance, we put the ramp on a hinge to be able to lift it up and easily remove the bin from the waste

• Install a hinge on the lower part of the ramp
• Attach the hinge to the box

6. Cutting of the holes on the box

In order to allow the entry of flies and the exit of larvae, holes must be drilled on the sides of the box.

For larvaes:

• Measure the location where the ramp arrives and count 2cm high for the opening
• Using a drill, make holes at the 4 ends of your opening
• Using a saw, connect these holes to create an opening

For the flies

• On the sides of the box, drill with a hole saw about ten openings of 5cm in diameter

7. Placing of the larvae collection box

• Using screws, fix the plastic box under the larvae exit slot

Make sure that the ramp is firmly fixed against the wall so that the larvae cannot fall back into the tank. If necessary, staple a bracket from the ramp to the collection box

8. Laying supports (if no separate aviary)

• Cut out pieces of cardboard boxes that are 10cm by 5cm thick.
• Assemble 4 pieces with string
• Attach two screws to the cleats at both ends of the box
• Hang a string on these screws
• Hang the egg-laying supports on the string so that they are as close as possible to the waste

The general recommendations for the design of the living environment are :

- Size your waste bin according to your weekly volume. Indeed, it is not necessary to have a too thick layer of substrate so it is better to be able to spread out your waste rather than stack it.

- Drill holes so that female flies can come and lay eggs inside.

- Place the egg-laying supports above the waste, as close as possible. These can be made of honeycomb cardboard or wooden planks separated by a pin (a gap must be left in which the flies will lay their eggs). During hatching, the larvae will fall directly into the waste.

- Have a well sealed waste box to prevent larvae from escaping in case of lack of food.

- Put a ramp for the exit of the larvae. It can be inclined up to 45 degrees but prefer a softer slope. As larvae tend to follow the edges of the box to find the exit, it may be good if the ramp is the full width of the box.

- Avoid the ventilation holes on top to protect the substrate as much as possible from rain.

Do not bring water into contact with eggs, they would burst!

On the pictures, you can see the plans of the system we had validated and that works on the boat. The fly inlet/outlet holes on the top have been plugged and the aviary attached to the cover has been changed to a separate aviary, see below.

Étape 2 - Construction of the aviary

If your BSF breeding project is carried out in a warm environment all year round with a natural presence of flies, this step is unnecessary. If, on the other hand, you think that flies would not come/remain naturally (like us on the boat), it is possible to create a closed-cycle system.

After testing a system where the mosquito net was directly above the box, we decided to build a separate aviary, mainly for waterproofing reasons. We were able to fill in the holes on top of our box and we can protect the flies and eggs in case of bad weather.

For the aviary construction:

Wooden frame:

• With a saw, cut the cleats and board to the right dimensions
• With screws, fix the 4 cleats of 80cm to the 4 corners of the board. Make sure they are all in the same direction.
• With screws, fix a 41cm cleat between two vertical cleats. Put its smallest side vertically, its width should perfectly fit the vertical cleats. It must be at the highest level, in the continuity of the vertical cleats.
• With screws, fix the second 41cm cleat.
• With angles, fix a 40cm strip between two vertical cleats. Put its smallest side horizontally. Its width should perfectly fit the vertical cleats. It must be at the highest level; in the continuity of the vertical cleats.
• Attach the second 40cm cleat.

Mosquito net and opening:

• Staple the end of the screen to a cleat, the entire height.
• Pull the screen by stretching it as far as it will go to the next strip.
• Staple it every time you pass a corner until you return to the starting point.
• Do the same on the top of the aviary
• Cut the board for the opening
• Attach two hinges to it
• Attach the two hinges on 2 vertical cleats (over their width)
• Cut the screen over the entire surface of the opening

Larvae management:

In the aviary, put:

• The egg-laying supports placed above a box in which waste is placed
• The box with the pupae in their substrate
• A box in which a cloth soaked in water will be placed, to be changed regularly.

Collect the larvae regularly and put the desired number in a box containing the dry substrate in which they will be buried. Adapt the size of the box according to the number of larvae recovered

Transfer the box to this space and let the transformation into a fly, reproduction and egg laying take place. Collect the egg-laying supports and hang them above the waste in the box or wait for them to hatch before putting them in.

Étape 3 - Life cycle of black soldier flies

Before you can set up a BSF farm, it is important to understand its life cycle.

The one is divided into 4 main phases:

• Larval stage

The larval stage is the only one where the BSF will seek to feed itself. This will be its only objective, in order to make a sufficient fat reserve to be able to transform into a pupae, then into a fly and reproduce. During its life, the larva takes 5000 times its initial mass (As if a baby reached the weight of an elephant in 2 weeks!!!). At this stage, the larvae are white in color and will grow from a few millimetres to 2.5 cm in length.

Its environment will be limited to the waste you give it, in which it will bury itself to feed itself.

This stage lasts on average between 14 and 16 days

For larvae, the optimal living conditions can be summarized as follows:

- Hot climate:the ideal temperature is between 24 and 30°C. If it is too hot, the larvae will crawl away from the food in search of a cooler place. If it is too cold, the larvae will slow their metabolism, eat less and develop more slowly.

- Shaded environment : larvae avoid light and always seek a shaded environment, away from sunlight. If their food source is exposed to light, they will move deeper into the food layer to escape the light.

- Moisture content : The food source must be very humid with a water content between 60% and 90% so that the larvae can ingest the substance.

• Pre-pupae

Once it has accumulated enough reserve, the larvae will transform into pre-pupae. At that time, it replaces its buccal part with a hook-shaped structure and becomes dark brown to anthracite grey. She will use this hook to get out of her wet environment to reach a dry, shaded and protected from predators to transform into a pupae.

It is therefore necessary to provide an exit ramp to a dry place, in which she can bury herself in order to initiate the pupation process.

• Pupae

Once in a suitable environment, the larva becomes a pupae, stops moving and is ready to turn into a fly. This process will take between two and three weeks.

• Fly

From the moment it hatches until its death, the BSF will have only one goal: to reproduce. She will live about 1 week and does not need to feed, only a source of water will be needed to keep her hydrated.

However, BSF need natural sunlight to reproduce and an optimal temperature between 25 and 32°C. Once they have found their partner, the females will look for a place to lay eggs. They particularly appreciate the gaps and the nests can be made of honeycomb cardboard for example. In addition, they will seek to lay eggs as close as possible to the food source so that as soon as they hatch, the larvae can feed.

Sunlight is a trigger for the fly mating cycle, so it is essential that the breeding site is well placed

The most delicate part remains reproduction, the life cycle of the fly being very short, the mating conditions must be met quickly if we want to obtain the next generations.

Étape 4 - Food

The larvae of the BSF can feed on most organic waste. They will degrade it to a greater or lesser extent. It is possible to add food only every two to three days, but if the larvae no longer have enough to eat, they will try to get out of the bin and look for another source of waste.

The larvae will feed mainly on low-fibrous waste (overripe fruit, vegetables, some leaves such as cabbage, etc.), so it is not necessary to put green waste (leaves, branches, grass). Similarly, thick fruit skins (banana, orange, lemon...) or pits will not be completely degraded. However, they will scrape off all the food available and it may be interesting to put them on, it will just not reduce the volume of waste.

It is also possible to give them animal or human faeces (droppings, dry toilets...). As larvae naturally destroy bacteria such as salmonella [1], it seems possible to feed them to animals without risk of transmission.

The optimal substrate conditions for larvae can be summarized as follows:

- Depth: "About ten centimeters. If there is more, the larvae will bury deeper and may not emerge. If there are fewer of them, they won't be able to bury themselves properly.

Nutrients: Protein-rich substrates and readily available hydrocarbons ensure good larval growth.

- Food aspect : The larvae do not have a chewing device, so access to nutrients will be easier if the substrate is composed of small pieces or even in liquid or pasty form.

-Frequency of filling: Always check that the larvae have enough food. If the larvae that are still white (not yet in the pre-pupae stage) are trying to extract themselves, this may be the cause.

- Humidity: 80% humidity is ideal. If the substrate is too dry they will not be able to assimilate the food well, if it is too wet they will try to go out to a drier place.

Ne pas mettre de marc de café, cela ferait mourir les larves !

Étape 5 - Larvae harvest

After 2 weeks of feeding on the waste, the BSF larvae can be harvested. At this stage, the larvae have reached their maximum weight, but have not yet transformed into pre-pupae. Their nutritional value is therefore at its highest. Harvesting is the process by which larvae are separated from the residue. This can be done by using a manual or automated screen that easily separates larvae from residues.

• Use a shovel to spread a layer of compost and larvae on the sieve. According to our tests, it is preferable to use a sieve with relatively large mesh sizes.
• Pour water over the spread layer to carry the larvae into the tank
• Once the bin is full, pour it into a box on which you will have previously installed a finer filter to recover the larvae (a net works very well).
• The larvae thus harvested can be fed directly to animals (poultry, fish...) or dehydrated (in a solar oven for example) and reduced to flour to preserve them.
• The organic waste residue can be dried in the sun and used as compost.

Notes et références

[1] Erickson, M. C., M. Islam, C. Sheppard, J. Liao, and M. P. Doyle. 2004. Reduction of Escherichia coli 0157:H7 and Salmonella enterica serovar Enteritidis in chicken manure by larvae of the black soldier fly. J. Food Protection. 67:685-690

https://www.eawag.ch/en/department/sandec/projects/mswm/black-soldier-fly-biowaste-processing/

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