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|Step_Title=<translate>Clônage du champignon</translate> | |Step_Title=<translate>Clônage du champignon</translate> | ||
− | |Step_Content=<translate>'''Le clônage''' est une étape délicate car elle nécessite un espace propre et stérile pour éviter les contaminations. Dans le cadre du projet, nous avons monté un ChampiLab, un atelier partagé où l'on retrouve tout le matériel nécessaire pour réaliser du clônage. Notamment, nous avons fabriqué un flux laminaire avec l'entreprise Breizh Bell, spécialiste de la culture de champignons dans une démarche low-tech (voir tutoriel Biosphère Urbaine - ChampiLab). '''Le clônage peut se réaliser avec toute variété de champignons. Dans le cadre du projet nous avons généralement clôner des pleurotes.''' | + | |Step_Content=<translate>'''Le clônage''' est une étape délicate car elle nécessite un espace propre et stérile pour éviter les contaminations. Dans le cadre du projet, nous avons monté un ChampiLab, un atelier partagé où l'on retrouve tout le matériel nécessaire pour réaliser du clônage. Notamment, nous avons fabriqué un flux laminaire avec l'entreprise Breizh Bell, spécialiste de la culture de champignons dans une démarche low-tech (voir tutoriel Biosphère Urbaine - ChampiLab). '''Le clônage peut se réaliser avec toute variété de champignons, idéalement pas trop vieux : un jeune champignon aura une croissance plus rapide. Dans le cadre du projet nous avons généralement clôner des pleurotes.''' |
<br /> | <br /> | ||
− | * Lorsque vous rentrez dans le ChampiLab, mettez une blouse de laboratoire, une charlotte sur la tête, un masque, des gants hygiéniques, des protections de chaussure | + | *Lorsque vous rentrez dans le ChampiLab, mettez une blouse de laboratoire, une charlotte sur la tête, un masque, des gants hygiéniques, des protections de chaussure (voir photo 1) |
− | * Nettoyez les surfaces de travail et le filtre du flux laminaire avec un tissu propre imbibé d'alcool à 70°C | + | *Nettoyez les surfaces de travail et le filtre du flux laminaire avec un tissu propre imbibé d'alcool à 70°C (voir photo 1) |
− | + | *Récupérez les blocs de boîte de Pétri avec des gants résistants à la chaleur et laissez-les refroidire devant le flux laminaire (voir photo 2) | |
− | * | + | *Pendant ce temps, allumez la lampe à alcool et nettoyez à nouveau vos gants, les surfaces de travail et aspergez le champignon d'alcool à 70°C (voir photo 3) |
− | * | + | *Prenez le pieds du champignon puis coupez-le en 2 dans le sens de la longueur avec vos doigts (voir photo 4). Une fois le champignon coupé en 2, ne garder qu’une moitié |
− | + | *Désinfectez le scalpel et passez sa lame dans la flamme afin de la stériliser (voir photo 4) | |
− | + | *A l’aide de la lame du scalpel, coupez un carré de 5 mm de côté dans le cœur du champignon sous le chapeau, au niveau du pied (voir photo 4) | |
− | * | + | {{Warning|La lame du scalpel ne doit pas toucher l’enveloppe externe du pleurote, celle-ci n’est pas stérile}} |
− | |||
− | * A l’aide de la lame du scalpel, | ||
− | ' | + | * Gardez le morceau de champignon sur le scalpel, ouvrez le couvercle d'une des boîtes de Pétri, et placez le morceau de champignon au centre de la gélose (voir photo 5) |
− | |Step_Picture_00=Biosph_re_Urbaine_-_Cl_nage_de_champignons_Capture_d_e_cran_2024-10- | + | * Refermez la boîte de Pétri et sceller avec le parafilm pour éviter les contaminations par l’air. Appliquez une extrémité du parafilm sur un bord de la boîte de Pétri puis tirez le parafilm pour le déposer sur toute la circonférence (voir photo 5). Il faut que le parafilm recouvre bien l'ouverture créée par le couvercle (voir photo 6) |
− | |Step_Picture_01=Biosph_re_Urbaine_-_Cl_nage_de_champignons_Capture_d_e_cran_2024-10- | + | * Placez vos boîtes de Pétri dans un environnement sombre et ventilé comme un placard. Le développement du mycélium prendra quelques jours |
+ | |||
+ | |||
+ | ''Question ? Comment on fait pour les autres boîtes de Pétri ? Il faut plsuieurs champignons ou pas ? Combien ?''</translate> | ||
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{{Notes | {{Notes | ||
− | |Notes=<translate></translate> | + | |Notes=<translate>'''Document rédigé par Emma Bousquet-Pasturel dans le cadre de programme de sciences participative de [https://www.biosphere-experience.org/ Biosphère Expérience].''' |
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{{PageLang | {{PageLang |
Tutorial de BIOSPHERE EXPERIENCE | Categories : Housing, Food
Ce tutoriel a été créé dans le cadre de l'expérience de mode de vie low-tech en ville menée par Biosphère Expérience à Boulogne Billancourt. Il présente les étapes de confection de mcyélium en grains par la filière dédiée à la culture de champignons.
Ce tutoriel a été créé dans le cadre de l'expérience de mode de vie low-tech en ville menée par Biosphère Expérience à Boulogne Billancourt. Il présente les étapes de confection de mcyélium en grains par la filière dédiée à la culture de champignons.
Le projet - Biosphère Urbaine
Pendant 4 mois, Corentin et Caroline ont expérimenté un mode de vie low-tech en ville en collaboration avec un réseau d'acteurs locaux. L'idée est de profiter de la forte densité de population pour répartir les connaissances, diviser le travail et mutualiser le matériel. Ainsi, Emma a constitué et coordonné des filières temporaires composées de structures locales mais aussi de citoyens. Au total, ce sont 14 personnes du territoire de Boulogne Billancourt qui ont participé à cette expérience !
Filière - culture de pleurotes
Les champignons sont délicieux et nutritifs, mais leur coût élevé sur le marché pose souvent un défi financier. Pour résoudre ce problème, Emma a créé une filière temporaire de culture de pleurotes qui assure tout le cycle de reproduction. À partir de pellets de paille et de mycélium en grains confectionnés au ChampiLab, Viriginie, Laurine, Corentin et Caroline ont reconstitué tout le cycle de culture du pleurote. L’idée est de mutualiser un espace pour créer des ateliers de confection des tours de culture mais aussi pour stocker le matériel et les tours pendant la phase d'incubation. Ce tutoriel vise à démocratiser la culture de pleurotes à l'échelle d'un territoire.
Déroulé de l'organisation durant l'expérience :
Ceci est une liste de matériaux exhaustive. Si vous avez déjà certains éléments, nous vous conseillons de favoriser la seconde main et d’adapter les dimensions et tailles tout au long du tuto.
Quantité pour réaliser 8 sacs de mycélium en grains ?
Ceci est une liste d'outils exhaustive à adapter en fonction de ce que vous possédez déjà.
Suivant le montage que vous choisissez et votre utilisation du réemploi, nous estimons entre 10 et 20 euros le coût pour réaliser un sac de mycélium en grains. Cela dépend de...
Avant de faire vos achats, nous vous conseillons fortement de lire en entier le tutoriel. Nous vous encourageons à réaliser des achats groupés pour éviter le gaspillage et partager les éventuels frais de livraison.
Pour rappel, ce tutoriel est prévu pour réaliser 3 sacs de 1,7 kg de mycélium en grains chacun. Adaptez les indications selons le nombre de sac que vous souhaitez réaliser.
Pour chacun de sacs de culture :
Stérilisation à la cocotte minute avec manomètre
Stérilisation à l'autoclave
Le clônage est une étape délicate car elle nécessite un espace propre et stérile pour éviter les contaminations. Dans le cadre du projet, nous avons monté un ChampiLab, un atelier partagé où l'on retrouve tout le matériel nécessaire pour réaliser du clônage. Notamment, nous avons fabriqué un flux laminaire avec l'entreprise Breizh Bell, spécialiste de la culture de champignons dans une démarche low-tech (voir tutoriel Biosphère Urbaine - ChampiLab). Le clônage peut se réaliser avec toute variété de champignons, idéalement pas trop vieux : un jeune champignon aura une croissance plus rapide. Dans le cadre du projet nous avons généralement clôner des pleurotes.
Question ? Comment on fait pour les autres boîtes de Pétri ? Il faut plsuieurs champignons ou pas ? Combien ?
Document rédigé par Emma Bousquet-Pasturel dans le cadre de programme de sciences participative de Biosphère Expérience.
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